Run Control_FreeC to calculate tumor purity

Installation ^[1]

wget https://github.com/BoevaLab/FREEC/archive/refs/tags/v11.6.tar.gz
tar -zxvf v11.6.tar.gz

cd PATH_TO/FREEC-11.6/src/
make

PATH_TO/FREEC-11.6/src/freec --help

Usage

Running Control-FREEC on a test data

Download a test dataset for HCC1143 and HCC1143-BL (from Chiang et al., 2009):

mkdir test_FREEC
cd test_FREEC
wget http://xfer.curie.fr/get/l4nQtrIsGmo/test.zip
PATH_TO/FREEC-11.6/src/freec -conf myConfig.txt -sample sample.bam -control control.bam
# Or
# PATH_TO/FREEC-11.6/src/freec -conf myConfig.txt (if BAM files are provided directly in the config file)

	/PATH_TO_FREEC/freec -conf config_ctrl.txt

to run it without a control sample (50kb window):
	/PATH_TO_FREEC/freec -conf config_GC.txt

to run it only for the normal sample (50kb window):
	/PATH_TO_FREEC/freec -conf config_BL.txt

Prepare input files

Generate a chromosome length file from a fasta file

samtools faidx your_file.fasta
cut -f1,2 your_file.fasta.fai | grep -w -E '(2R|2L|3R|3L|X|Y|4)' > filtered_chromosomes.txt

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1. http://boevalab.inf.ethz.ch/FREEC/tutorial.html ↩︎